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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/01/2005 |
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Data da última atualização: |
03/08/2017 |
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Autoria: |
GRANGE, L.; HUNGRIA, M. |
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Título: |
Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. MenosAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo |
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Palavras-Chave: |
Rizóbio. |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
LEADER 02618naa a2200157 a 4500
001 1467760
005 2017-08-03
008 2004 bl --- 0-- u #d
100 1 $aGRANGE, L.
245 $aGenetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems.
260 $c2004
520 $aAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena.
650 $aFeijão
653 $aRizóbio
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 36, n. 9, p. 1389-1398, Sept. 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 552 | |
| 123. |  | OLIVEIRA, M. C. N. de; CARRÃO-PANIZZI, M.C.; MANDARINO, J. M. G. Método de componentes principais na avaliação de saponinas em cultivares de soja. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 242, trab. 442. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 127. | | QUEIROZ, E. F. de; SILVA, R. J. B. da; OLIVEIRA, M. C. N. de. Modelo de analise de regressao periodica da precipitacao mensal, da bacia atlantico sudeste, no Estado do Parana. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 36, n. 5, p. 727-42, maio 2001 Título em inglês: A model for monthly rainfall periodic regression analysis, of the southeast Atlantic basin, on Paraná State.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Soja; Embrapa Unidades Centrais. |
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| 132. | | BASSOI, M. C.; OLIVEIRA, M. C. N. de; TAVARES, L. C. V. Eficiência dos fungicidas metconazol e da mistura comercial piraclostrobina + epoxiconazol no controle de doenças do trigo. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 4., 2010, Cascavel. Ata e resumos... Cascavel, PR: Comissão Brasileira de Pesquisa de Trigo e Triticale: COODETEC, 2010. 1 CD-ROM. Organizado por Volmir Sergio Marchioro, Francisco de Assis Franco, Andrelise Daltoé. 5 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 133. | | CARRAO-PANIZZI, M.C.; PRUDENCIO-FERREIRA, S.H.; KITAMURA, K.; OLIVEIRA, M. C. N.; BELEIA, A. Efeito dos teores de isoflavonoides no sabor do extrato soluvel e graos cozidos de soja. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO SUL, 24., 1996, Pelotas. Ata e resumos. Pelotas: EMBRAPA-CPACT, 1997. p.194. (EMBRAPA-CPACT. Documentos, 30).| Biblioteca(s): Embrapa Soja. |
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| 134. | | BASSOI, M. C.; FRONZA, V.; OLIVEIRA, M. C. N. de; HIRAKURI, M. H.; TAVARES, L. C. Eficiência da mistura comercial estrobilurina + triazol (trifloxystrobin + tebuconazole) no controle de doenças do trigo: II - Ponta Grossa, PR. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 3., 2009, Veranópolis. Ata e resumos... Veranópolis: Comissão Brasileira de Pesquisa de Trigo e Triticale: FEPAGRO: ASAV; Passo Fundo: Embrapa Trigo, 2009. 3 p. Subcomissão Fitopatologia, trab. 93. 1 CD-ROM. Organizado por Ricardo Lima de Castro, Simone Lancini, André Dabdab Abichequer, Bernadete Radin, Bruno Lisboa, Douglas Lau, Fabiano Paganella, João Leonardo Fernandes Pires, João Rodolfo Guimarães Nunes, Luciano Kayser Vargas, Nelson Gomes Bertoldo. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 135. | | BASSOI, M. C.; FRONZA, V.; OLIVEIRA, M. C. N. de; HIRAKURI, M. H.; TAVARES, L. C. Eficiência da mistura comercial estrobilurina + triazol (trifloxystrobin + tebuconazole) no controle de doenças do trigo: I - Londrina, PR. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 3., 2009, Veranópolis. Ata e resumos... Veranópolis: Comissão Brasileira de Pesquisa de Trigo e Triticale: FEPAGRO: ASAV; Passo Fundo: Embrapa Trigo, 2009. 3 p. Subcomissão Fitopatologia, trab. 90. 1 CD-ROM. Organizado por Ricardo Lima de Castro, Simone Lancini, André Dabdab Abichequer, Bernadete Radin, Bruno Lisboa, Douglas Lau, Fabiano Paganella, João Leonardo Fernandes Pires, João Rodolfo Guimarães Nunes, Luciano Kayser Vargas, Nelson Gomes Bertoldo. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 139. | | GAUDENCIO, C. de A.; WOBETO, C.; OLIVEIRA, M. C. N. de; REICHERT, P. Influencia da rotacao de culturas, sobre o estado de agregacao do solo, em Guarapuava, PR. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e resumos. Londrina: EMBRAPA-CNPSo, 1998. p.193-194. (EMBRAPA-CNPSo. Documentos, 121).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 552 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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